The research of possible biochemical and morphological markers of “no-reflow” phenomenon in experiment
Abstract
Aim – to research the interrelation between the carbonyl derivatives of proteins and damage
of vascular endothelium as potential predictor of the development of «no-reflow» phenomenon
in experimental reperfusion injury.
Methods. Experimental study was carried out on Wistar rates according to the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific
Purposes (Strasbourg, 18.03.1986) and the Order of the Ministry of Healthcare of the Russian
Federation «On the ratification of the rules of laboratory practice» N 267 iss. 19.06.2003. The
model of reperfusion injury has been performed by cross clamping of the aorta with following
conditioning. The activity of lysosomal cysteine proteases, oxidative modification of serum
proteins, level of homogenates in the affected vascular wall and intact vascular wall distal to
the manipulations were then evaluated. We used transmission electron microscopy the Carl
Zeiss Libra 120 (Germany). Statistical evaluation of data was performed according to the relevant methods of mathematical analysis provided by the advanced analytics software package
Statistica 10.0. Statistical significance was assessed with the Mann–Whitney U-test.
Results. In experimental reperfusion injury model oxidative stress developed from day 1 to
day 7 in serum and by day 3 to day 7 in vascular wall. Oxidative stress in vascular wall has been
characterized by the attenuation of adaptive reserve potential and predominance of secondary
markers with activity of cathepsins B and L reaching its maximum levels at days 3 and 5, which
corresponded to the structural changes in vascular intima.
Conclusion. Increased levels of secondary metabolites of the oxidative stress in vascular wall
lead to the damage of endothelial cells, which may determine an important structural element
in the pathogenesis of the «no-reflow» phenomenon.
Keywords:«no-reflow» phenomenon, reperfusion, oxidative stress, carbonyl derivatives of proteins, cathepsins, endothelium
Clin. Experiment. Surg. Petrovsky J. 2018; 1 (19): 62–69.
DOI: 10.24411/2308-1198-2018-00009
Received: 16.08.2017. Accepted: 25.01.2018.
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