Testing of additive materials in human fibroblast cell cultures

Relevance. To fully restore the functionality of the hand after surgery or trauma, it is required to repeat the anatomy of the lost segments of the hand as accurately as possible. This ambitious task is possible with the use of the 3D-modeling and additive technologies.

The aim of the study is testing of additive materials obtained from a titanium powder of grade VT1-00 by laser sintering on cell cultures of human fibroblasts. Within the framework of the goal, the following tasks were solved: determination of the cytotoxicity of the presented materials; determination of the influence of the studied materials on the proliferative activity of dermal fibroblasts in culture; determination of the adhesion of dermal fibroblasts to the materials studied. The study is prospective, evidence level II.

Material and methods. For the study, samples of the additive material obtained from the titanium powder of grade VT1-00 by laser sintering were presented. The testing was carried out on the culture of human 7th passage dermal fibroblasts according to the interstate standard by the method of direct contact (GOST ISO 10993-5-2011).

Result. Testing of samples of additive material on adhesiveness has demonstrated that the number of cells detected by attached to the surface of the test material is not less than 97% (compared to the control group – 81.5%). We noted the absence of cytotoxicity of the materials studied as a result of the LDH test. There were no noticeable changes in the morphofunctional characteristics of dermal fibroblasts in the presence of samples of additive titanium. A good proliferative activity of cells in the presence of test materials was also noted. In this case, non-viable cells were less than 5% at all times of the experiment.

Conclusion. Thus, testing of the submitted samples on the culture of human dermal fibroblasts by direct contact method according to the recommendations of the interstate standard GOST ISO 10993-5-2011 showed the absence of cytotoxicity, high proliferative potential and preservation of cell viability. These results are part of the preclinical test of the additive material for the manufacture of personalized endoprosthesis of the hand joints.

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