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1 . 2023

Experimental modeling of critical lower limb ischemia

Abstract

Background. For decades, cardiovascular diseases have remained one of the main causes of death in the world. A significant part of them is the lesion of the main and peripheral vessels. Critical ischemia of the lower extremities (CILЕ) occurs in 500–1000 cases per 1 million population. At the same time, pharmacological therapy of critical ischemia is an integral part of complex treatment, and if surgical intervention is impossible, it plays a dominant role.

In order to be able to study various approaches in the field of pharmacotherapy, it is necessary to use an adequate surgical model of critical ischemia of the hind limbs.

Aim. Working out and putting into practice the model of CILE.

Material and methods. The study used 50 mice (25♂ and 25♀) of the BALB/c line, aged 8–10 weeks. In the course of the experiment, surgical modeling of CILE and evaluation of tissue perfusion of the ischemic hind limb were performed. On the 48th day after the CILE simulation, pathomorphological and histological analysis of the hind limb of the animals was performed. According to the results of the study, the CILE model was worked out. This model shows the effectiveness of the drug VEGF165. The scheme and dosage of the use of this drug has been worked out.

Conclusion. As a result of the experiment, a working model of CILE was formed, which in the future can be widely used in preclinical practice to evaluate the effectiveness of new drugs being developed, as well as treatment regimens for critical lower limb ischemia in complex therapy.

Keywords:critical lower limb ischemia (CILE); experimental modeling; recombinant endothelial growth factor (VEGF165)

Financing. The study was carried out with the financial support of JSC “Generium”.

Conflict of interest. The authors declare that there is no conflict of interest.

For citation: Ovsepyan A.A., Belyanina E.V., Bocharova E.N., Borzov A.A., Katorkina E.I., Aksenova M.S., Lykov M.V. Experimental modeling of critical lower limb ischemia. Clinical and Experimental Surgery. Petrovsky Journal. 2023; 11 (1): 70–6. DOI: https://doi.org/10.33029/2308-1198-2023-11-1-70-76  (in Russian)

References

1.     Takako Goto T., Fukuyama N., Aki A., Kanabuchi K., Kimura K., Taira H., et al. Search for appropriate experimental methods to create stable hind-limb ischemia in mouse. Tokai J Exp Clin Med. 2006; 31: 118–32.

2.     Francki A., Labazzo K., He S., Baum E.Z., Abbot S.E., Herzberg U., et al. Angiogenic properties of human placenta-derived adherent cells and efficacy in hindlimb ischemia. J Vasc Surg. 2016; 64 (3): 746–56.e1.

3.     Padgett M.E., McCord T.J., McClung J.M., Kontos C.D. Methods for acute and subacute murine hindlimb ischemia. J Vis Exp. 2016; 112: 54166. DOI: https://doi.org/10.3791/54166  

4.     Skjeldal S., Grogaard B., Reikeras O., Muller C., Torvik A., Svindland A. Model for skeletal muscle ischemia in rat hindlimb: Evaluation of reperfusion and necrosis. Eur Surg Res. 1991; 23: 355–65.

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CHIEF EDITOR
CHIEF EDITOR
Sergey L. Dzemeshkevich
MD, Professor (Moscow, Russia)

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