Experimental modeling of critical lower limb ischemia
Abstract
Background. For decades, cardiovascular diseases have remained
one of the main causes of death in the world. A significant part of them is the
lesion of the main and peripheral vessels. Critical ischemia of the lower
extremities (CILЕ) occurs in 500–1000 cases per 1 million population. At the same time,
pharmacological therapy of critical ischemia is an integral part of complex
treatment, and if surgical intervention is impossible, it plays a dominant
role.
In order to be able
to study various approaches in the field of pharmacotherapy, it is necessary to
use an adequate surgical model of critical ischemia of the hind limbs.
Aim. Working out and putting into practice the model of
CILE.
Material and methods. The study used 50 mice (25♂ and 25♀) of the BALB/c
line, aged 8–10 weeks. In the course of the experiment, surgical modeling of
CILE and evaluation of tissue perfusion of the ischemic hind limb were
performed. On the 48th day after the CILE simulation, pathomorphological
and histological analysis of the hind limb of the animals was performed.
According to the results of the study, the CILE model was worked out. This
model shows the effectiveness of the drug VEGF165. The scheme and
dosage of the use of this drug has been worked out.
Conclusion. As a result of the experiment, a working model of
CILE was formed, which in the future can be widely used in preclinical practice
to evaluate the effectiveness of new drugs being developed, as well as
treatment regimens for critical lower limb ischemia in complex therapy.
Keywords:critical lower limb ischemia (CILE); experimental modeling; recombinant endothelial growth factor (VEGF165)
Financing. The study was carried out with the financial support
of JSC “Generium”.
Conflict of interest. The authors declare that there is no conflict of
interest.
For citation: Ovsepyan A.A., Belyanina E.V., Bocharova E.N., Borzov
A.A., Katorkina E.I., Aksenova M.S., Lykov M.V. Experimental modeling of
critical lower limb ischemia. Clinical and Experimental Surgery. Petrovsky
Journal. 2023; 11 (1): 70–6. DOI: https://doi.org/10.33029/2308-1198-2023-11-1-70-76
(in Russian)
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