The role of Roncoleukin in the correction of reactive changes of the small intestine (an experimental model)

• Ishunina T.A.
• Goncharov I.M.
• Polyansky M.B.
• Nazarenko P.M.
• Nazarenko D.P.

Abstract

Background. “Traditional” cholecystostomy with subsequent gallbladder mucoclasia minimizes surgical and anesthetic risks in elderly and senile patients with acute calculous cholecystitis, whereas postoperative administration of Roncoleukin allows to increase the cellular immunity in this category of patients. At the same time, the effectiveness of Roncoleukin in relation to cellular indicators of local immunity of the small intestine located nearby remains unexplored.

Аim – to investigate the reaction of various tissue elements of the small intestine to the inflammatory process induced by experimental modeling of gallbladder mucoclasia and the effect of Roncoleukin on these changes.

Material and methods. The study was performed on the 21 rabbit, that were divided into 3 groups with 7 animals each, according to the timing of withdrawal from the experiment on the 7^th, 14^th and 21^st days. In each group, 2 subgroups were identified: a control group in which gallbladder mucoclasia was simulated without the introduction of Roncoleukin, and an experimental one in which 25,000 IU of the drug “Roncoleukin” was administered to rabbits on the 1^st, 3^rd and 5^th days after surgery. Histological examination of fragments of the thymus, lymph nodes and small intestine was performed.

Results. In the small intestine, there was a change in the density of lymphocytes. The reaction of the integumentary epithelium, neurons of the intermuscular plexus and of the muscularis externa was noted as well. The use of Roncoleukin significantly increased the size of small intestinal lymphocytes at all periods of the experiment.

Conclusion. Surgical interventions on the gallbladder in rabbits lead to reactive changes in the small intestine, proceeding in logical connection with the dynamics of the inflammatory process. The introduction of Roncoleukin activates lymphocytes of the small intestine, which can contribute to the relief of inflammation and faster recovery following surgery.

Keywords:gallbladder mucoclasia; cholecystitis; small intestine; lymphocytes; epithelium

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