Histological and molecular analysis of the left ventricular aneurysm wall

• Popov M.A.
• Andreeva E.R.
• Gurevich L.E.
• Zybin D.I.
• Ratushny A.Yu.
• Matveeva D.K.
• Abdullaev S.A.
• Radchenkova O.V.
• Buravkova L.B.
• Shumakov D.V.

Abstract

Hibernating myocardium, potentially capable of restoring its function, is of clinical and scientific interest. Histological and molecular analysis of the hibernating myocardium in the zones of hypokinesis and normokinesis in a patient with ischemic heart disease was performed.

Material and methods. A molecular and immunohistochemical study of left ventricular myocardial biopsies was performed in a patient after surgical reconstruction of the left ventricle in combination with surgical revascularization.

Results. Morphological examination revealed a violation of the structure of cardiomyocytes, which correlates with the accumulation of matrix metalloproteinase type 9 in the cytoplasm of cardiomyocytes in these zones against the background of partial or complete destruction of their basement membranes formed by type IV collagen.

Analysis of the differential expression of genes encoding extracellular ar matrix proteins and matrix-associated molecules revealed a shift in the transcription profile due to a decrease in the expression of collagen genes, matrix proteases, and cell-matrix interaction molecules.

Conclusion. Based on the analysis, it can be concluded that the hibernating myocardium, as a result of the destruction of the structure of the sarcormers and basal membranes of cardiomyocytes, a decrease in the activity of extracellular matrix genes, is not able to further provide contractile function, and viable cells that are detected during morphological examination probably function only as a protective mechanism during early scar formation.

Keywords:ischemic heart disease; left ventricular aneurysm; left ventricular remodeling; hibernating myocardium; histological analysis; extracellular matrix; differential gene expression; reverse transcription polymerase chain reaction

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